Etribby3: Created page with "== Spring 2021 (2nd Semester) == ---- === '''Week One''' === ==== VIP Group Meeting (1/19/2021) ==== -Introductions and subgroup presentations -Intereste..."

2021-05-04T15:21:04Z

Created page with "== Spring 2021 (2nd Semester) == ---- === '''<big>Week One</big>''' === ==== VIP Group Meeting (1/19/2021) ==== -Introductions and subgroup presentations -Intereste..."

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== Spring 2021 (2nd Semester) ==
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=== '''<big>Week One</big>''' ===

==== VIP Group Meeting (1/19/2021) ====
-Introductions and subgroup presentations

-Interested in Liquid Waste Nitrification, Air quality, and greywater.
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=== '''<big>Week Two</big>''' ===

==== VIP Group Meeting (1/26/2021) ====
-Subgroup Decision & Subgroup Breakout (Liquid Waste Nitrification)

- Set group meeting time for Sunday at 12:00 PM on Bluejeans

- Possibly with hold experiment in Kendeda VIP lab or Ecology lab.

- Semester goal: scale up last semester's experiment

==== Sunday Meeting (1/31/2021) ====
-Prepped for this week's presentation

- Updated/edited Google Slides presentation

- Got access to Google drive/one note

-Brought up future questions:
* Budget for this semester?
* Need to complete Lab safety 101, RTK, Biosafety
-Future Goals:
* Determine a regular meeting time
'''Tuesday (2/2/2021)''' - Finished Lab safety 101, RTK, and General Biosafety trainings
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=== '''<big>Week Three</big>''' ===
==== VIP Group Meeting (2/2/2021) ====
- Weekly Presentations - first presentation!
-

-Presented our past achievements and future goals/ plans for this semester

- Feedback

- Budget= $125

- consider other factors that would affect the metabolism of the bacteria to make it more efficient

- consider community of microorganisms found in the system

- campus is a small sample

- see if there is existing literature on conditions of community

- vary variables like temperature, level of bubbles etc.

- cannot use real waste still, due to COVID protocols

- Because team is so large, consider scaling up even more/ doing two groups

- Materials

- 500 gallon plastic tub because leachate tank is plastic

- last semester supplies in a stock room in Boggs

- get access to Kendeda

==== Post Tuesday Response Meeting (2/5/2021) ====
- Semester Goals

- Finish research paper

- use a larger scale tank for experiment to better mimic leachate tank

- Preform lit review on Phosphate use in the nitrification process

- Get Phase III proposal approved

- Create a materials list.

- determine size of tank needed for statistically significant results.
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=== '''<big>Week Four</big>''' ===

==== VIP Group Meeting (2/9/2021) ====
- We did not present this week

==== Mini Group Meeting (9/12/2020) ====
- Long Term Goal: engineer a redesign of the current waste management system in the Kendeda Building
*Currently, just a plastic tub,
*Want to propose a R&D
- Short Term
* w/in a month (March 1st) Project Proposal
* Design set up, standard procedure, materials list, operating cost list
* < $250
- Phase III
* Per Dr. Glass recommendation, we are looking into phosphate extraction, as it is in high demand but low supply
* there are natural phosphates in liquid waste and if we can extract them
** makes our process economically favorable, which will incentivize its use.
* Sub teams
** Project Proposal: Maria and Shalini
** Phosphate and general Research: Mia, Sam and myself
* Goals
** investigate phosphate cycle as phosphate is extracted through crystallization and erosion, we want to streamline if possible
** find a way to manage pH as phosphate is accepts hydrogens from the water to stabilize itself
** need buffer?
** Create a comprehensive list of acid base reactions, catalysts etc. done by March
* To do (me)
** determine who w/in sub team is going to be focused on phosphate and who will focus on general
** Email Dr. Glass
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=== '''<big>Week Five</big>''' ===

==== VIP Group Meeting (2/16/2021) ====
-Presentation Day

- presented our phase III set up

- introduced our additional focus of the semester (Phosphate retrieval)

- explained how we broke team into two subteams

- budget shouldn't be an issue

- Questions from the panel

- where would we get the phosphorus from because it isn't present in our current solution of urea

- suggested we try animal manure (cow, elephant)

- Goals:

- get solidified materials list

- research phosphate retrieval and consider a experiment

- create an educational video

- enter VIP competition

Weekly Meetings: Friday's at 3:00 PM

'''Mini Group Meeting (2/19/2021)'''

- we calculated the materials needed for our scaled up experiment

-32.22 g TAPSO buffer

- 0.54 g Manure

- 110.76 g Urea

- we created a procedure

- 6 2L tubs

- 3 with just urea mixture, 3 with manure urea mixture

New Information:

- The sludge in the leachate tank is semi solid

- phosphate retrieval will be difficult, so continue with lit reviews

- We do not know the chemical makeup of the leachate tank

Questions for Leavey:

- Will we need a BSL 2 lab because we are working with cow manure?

Goals:

- Short term:

-Create phase III document by Monday, 22nd

- visit Kendeda building

- Long term:

- verify conclusions from Phases I and II

- eliminate errors in data from Phases I and II

- track the life cycles of the bacteria

- enter VIP competition

'''Sunday (2/21/2021)'''
* Created and edited Phase III doc
* Created Phase III operating procedure outline
* Confirmed meeting time with Dr. Glass
* Sent Phase III doc to Aravind and Maria to get suggestions
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=== '''<big>Week Six</big>''' ===

==== VIP Group meeting (2/23/2021) ====
- We did not present today

- I did research on phosphate recovery based on Dr. Glass' suggestions and resources.

- Added to the lit review.

==== Subgroup Meeting (2/26/2021) ====
- I am now in the nitrification sub-team
* We got a lot of research about phosphate retrieval done
-
- - use MgCl and a stirred reactor to precipitate struvite

- best pH is between 8.5-10

- consider how this high pH will affect bacteria
* Wrote and sent Leavey the Phase III document. Also sent Shalini's literature review.
* made procedure outline
* Formatted presentation for next week.
----

=== '''<big>Week Seven</big>''' ===

==== VIP Group meeting (3/2/2021) ====
- Weekly Presentation
* gave presentation explaining the Phase III doc and our new research
* Presented a possible experiment design for phosphate retrieval
* TAPSO is ordered
FeedBack:
* Talk to Dr. Grantham about phosphate precipitation
* Talk to steve place about fertilizer economics

'''Lab Meeting (Friday 3/5/2021)'''
* Sam informed us of the information that Dr. Grantham gave him.
* We determined additional materials that we'd need
** strainer-- home depot
** 64+ oz plastic containers--ikea
** aluminum foil-- Kroger
** Maria and Shalini went to kendeda (we have access via backdoor)
*** couldn't find prep room with materials
*** put the supplies that could be found in Kendeda 280
* We got information about phosphate precipitation from Dr. Tang
* Hoping to get in the lab in one week.
* Emailed Marlon Ellis (Kendeda Manager) about separation of waste and content of foam
** foam is from Clivus Multrum
* Goals
** Create a spreadsheet of lab assignments.

----

=== '''<big>Week Eight</big>''' ===

==== VIP Group meeting (3/9/2021) ====
* We did not present this week
* Asked Leavey if she could order manure, paint strainer, and containers
** got black 1 gal containers so we don't need aluminum
* Rest of supplies are in Kendeda now
* Goals:
** Get in Lab next week
** finish lab schedule
'''Mini Group Meeting (3/12/2021)'''
* Mia and I went to Kendeda and got a tour from Mr. Ellis
* Picked up soap sample (need more)
* Learned that waste gets transported with the help of gravity through pipes to clivus composters
* once in the composter, woodchips are added and the solid waste decomposed (over ten years)
* this mixture of liquid and solid waste is strained and pumped into the leachate tanks
* once over 3/4 full (1000 gallon) waste management service in Cartersville picks it up and recycles its nutrients

=== '''<big>Week Nine</big>''' ===
==== VIP Group meeting (3/16/2021) ====
- Wellness day so no class

'''Material gathering (3/20/2021)'''
* Maria and I gathered all the materials to start the experiment
* created the urea solution and put the manure samples into the respective beakers.

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=== '''<big>Week Ten</big>''' ===
'''Mini Group Meeting (3/22/2021)'''
* day 0, calibrated probes and completed VIP video
* added buffer solution

==== VIP Group meeting (3/23/2021) ====
* Presented findings from tour
* explained Phase III experimental set-up group
* updated on phosphate research
'''Continue ever other day measurements (3/24/2021)'''
* Shalini and I took measurements
* Still concerned about how pH is affected by the soap
'''Continue every other day measurements (3/26/2021)'''
* Meet with Tang
* Confirmed our previous findings
* She suggested that we consider incubation of some sort
----

=== '''<big>Week Eleven</big>''' ===
==== VIP Group Meeting (3/30/2021) ====
* did not present this week
'''Week Eleven Subgroup meeting (4/2/2021)'''

-*began our experiment (without bacteria or bubblers)
* having a difficult time calibrating our nitrate and ammonium probes
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=== '''<big>Week Twelve</big>''' ===
==== VIP Group Meeting (4/6/2021) ====
* Presented this week, explained our issues (bubblers creating foam in beakers with soap)
* Dr. Grantham suggested magnetic stir bars and plates because it will still oxygenate but without all the excess foam
* Ph is raised significantly by the addition of soap (makes sense since soap is a base)
----

=== '''<big>Week Thirteen</big>''' ===
====VIP Group Meeting (4/13/2021)====
-Weekly Presentation
* did not present this week
* in smaller group, we discussed the issues with our probes (it appears despite length or redundancy of calibrations, readings are off)
* discussed the process for the rest of the experimentation
* should be done next week and can start compiling data
-

====Weekly Meeting ====
----* met to discuss VIP competition
* analyzed data from trial
* discussed shortcomings of the trial/ ideas for the future
* created our final presentation

=== '''<big>Week Fourteen</big>''' ===
====VIP Group Meeting (4/20/2021)====
----
* final presentations
* gave a breakdown of the background of our experiment
* explained our process
* communicated our findings from this phase
*pH increases with soap (base) but all pH are generally stable enough for nitrification to occur
*pH increases over course of reaction
*Irregularities with ammonium probe inhibited further data comparison as ammonium concentration helps provide context for the course of reaction

=== '''<big>Week Fifteen</big>''' ===
====VIP Group Meeting (5/4/2021)====
- we did not present
- we won third place for our poster
- we won first place (and $1000) for our project in the people and environment category.

Emily's Notebook - Revision history

Etribby3: Created page with "== Spring 2021 (2nd Semester) == ---- === '''Week One''' === ==== VIP Group Meeting (1/19/2021) ==== -Introductions and subgroup presentations -Intereste..."